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IL-35 ELISA Kit, Human

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  •  This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-35. The Human IL-35 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

    Interleukin 35 (IL-35) is an IL-12 family cytokine produced by regulatory, but not effector, T-cells and plays a role in immune suppression. It is a dimeric protein composed of IL-12α and IL-27β chains, which are encoded by two  separate genes called IL12A and EBI3, respectively. Secreted by regulatory T-cells (Tregs), IL-35 suppresses inflammatory responses of immune cells. IL-35 is not constitutively expressed in tissues, but the gene encoding IL-35 is transcribed by vascular endothelial cells, smooth muscle cells and monocytes after activation with proinflammatory stimuli.  Studies in mice show the absence of either IL-35 chain from regulatory Tregs reduces the cells’ ability to suppress inflammation; this has been observed during cell culture experiments and using an experimental model for inflammatory bowel disease. To produce its suppressive effects, IL-35 has selective activities on different T-cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cellpopulations.

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